منابع مشابه
Splicing-Dependent RNA Polymerase Pausing in Yeast
In eukaryotic cells, there is evidence for functional coupling between transcription and processing of pre-mRNAs. To better understand this coupling, we performed a high-resolution kinetic analysis of transcription and splicing in budding yeast. This revealed that shortly after induction of transcription, RNA polymerase accumulates transiently around the 3' end of the intron on two reporter gen...
متن کاملA global change in RNA polymerase II pausing during the Drosophila midblastula transition
Massive zygotic transcription begins in many organisms during the midblastula transition when the cell cycle of the dividing egg slows down. A few genes are transcribed before this stage but how this differential activation is accomplished is still an open question. We have performed ChIP-seq experiments on tightly staged Drosophila embryos and show that massive recruitment of RNA polymerase II...
متن کاملIn vivo transcriptional pausing and cap formation on three Drosophila heat shock genes.
The regulation of many eukaryotic genes occurs at the level of transcriptional elongation. On the uninduced hsp70 gene of Drosophila melanogaster, for example, an RNA polymerase II complex has initiated transcription but has paused early in elongation. In this study, we examine pausing on hsp70 and two of the small heat shock genes (hsp27 and hsp26) at high resolution, using a technique that ut...
متن کاملMisincorporation by RNA polymerase is a major source of transcription pausing in vivo
The transcription error rate estimated from mistakes in end product RNAs is 10-3-10-5 We analyzed the fidelity of nascent RNAs from all actively transcribing elongation complexes (ECs) in Escherichia coli and Saccharomyces cerevisiae and found that 1-3% of all ECs in wild-type cells, and 5-7% of all ECs in cells lacking proofreading factors are, in fact, misincorporated complexes. With the exce...
متن کاملRNA Polymerase Pausing during Initial Transcription
In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied initial transcription on a lac promoter using single-molecule fluorescence observ...
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ژورنال
عنوان ژورنال: Nature
سال: 2007
ISSN: 0028-0836,1476-4687
DOI: 10.1038/nature06324